Scientist Networking And Profiling (SNAP)

For complete information please visit our submission site at:

https://my.ilabsolutions.com/service_center/show_external/3122/BiomolecularCoreLab

Services Offered

Operating under strict HIPAA regulations, we are a CLIA-certified site. Our service core unit currently supports the Nemours Molecular Diagnostic DNA sequencing and fragment analysis CLIA-certified tests. We also provide backup services to other cores in the Delaware Valley Network, including DNA sequencing coverage services for the University of Delaware DNA Sequencing and Genotyping Core.

Expression Analysis:

Affymetrix microarray (global gene expression, Transcriptome, 3’IVT and WT assays) Pathway-focused real-time qPCR (mRNA and miRNA)

Genotyping/Variant Analysis:

• Digital PCR (absolute/relative quantification, CNV analysis)
• Allelic discrimination probes (SNP real-time PCR)
• Affymetrix microarray (CNV, CytoScan, SNP arrays)
• Fragment analysis (capillary electrophoresis up to 1200 bp)
• DNA sequencing (Sanger sequencing)
• Next Generation DNA Sequencing (Ion PGM)
• NGS Library Preparation (Amplicon libraries, fragmentation libraries)

Shared Equipment:

• Agilent Bioanalyzer Lab-On-A-Chip
• Beckman Biomek 3000 Liquid Handler
• NanoDrop 2000
• ABI7900 384-Well Real-Time Genetic Analyzer
• Affymetrix GeneChip System 3000
• PCR Tamer
• Thermocyclers
• Pippen Prep

BCL has a purchasing agreement with Integrated DNA Technologies (IDT) to obtain high quality oligonucleotides at reduced cost. The turn around time for standard primer is two days. Modifications such as fluorescent labeling/HPLC purification or dual (Real-Time) probes require 5-7 days for synthesis. Oligo's can be picked up at the core facility during business hours (M-F 9:00 AM - 4:00 PM).

Please contact the assistant director concerning the Oligo ordering information.

For general IDT information please visit: www.idtdna.com

To access Nemours oligo ordering portal go to: www.idtdna.com/NEMOURW-BioMed

Mitch Gore, IDT sales consultant.  Phone:610-524-1350  
Please contact the Biomolecular Core Director, Katia Sol-Church, PhD. (via phone (302-651-6705) or by email.) if you would like his email address.

Operating under strict CLIA guidelines, BCL sequencing services currently supports laboratories offering diagnostic tests for the following diseases:

• Barth Syndrome
• Benign Hereditory Chorea
• Costello Syndrome
• Cranio-Facial-Cardio Syndrome
• Duchenne Muscular Dystrophy
• Fiengold Syndrome
• Pelizaeus-Merzbacher Disease
• Pelizeaus-Merzbacher-Like Disease
• Rett syndrome
• Spinal Muscular Atrophy

Consultations on project design for hypothesis-based research and for the development of new molecular diagnostics tools are ongoing. Quotes are available upon request for project's budget developments. Interested individuals should set up an appointment by contacting the laboratory director, Dr. Katia Sol-Church via phone (302-651-6705) or by email.