Differential effects of substrate modulus on human vascular endothelial, smooth muscle, and fibroblastic cells

TitleDifferential effects of substrate modulus on human vascular endothelial, smooth muscle, and fibroblastic cells
Publication TypeJournal Article
Year of Publication2012
AuthorsRobinson, K. G., Nie T., Baldwin A. D., Yang E. C., Kiick K. L., & Akins R. E.
JournalJournal of biomedical materials research. Part A
Date Published2012 May
KeywordsAnimals; Cell Adhesion; Cell Proliferation; Cell Shape; Cluster Analysis; Elastic Modulus; Fibroblasts; Gene Expression Regulation; Human Umbilical Vein Endothelial Cells; Humans; hydrogel; Mice; Muscle, Smooth, Vascular; Myocytes, Smooth Muscle; NIH 3T3 Cells; Oscillometry; Rheology

Regenerative medicine approaches offer attractive alternatives to standard vascular reconstruction; however, the biomaterials to be used must have optimal biochemical and mechanical properties. To evaluate the effects of biomaterial properties on vascular cells, heparinized poly(ethylene glycol) (PEG)-based hydrogels of three different moduli, 13.7, 5.2, and 0.3 kPa, containing fibronectin and growth factor were utilized to support the growth of three human vascular cell types. The cell types exhibited differences in attachment, proliferation, and gene expression profiles associated with the hydrogel modulus. Human vascular smooth muscle cells demonstrated preferential attachment on the highest-modulus hydrogel, adventitial fibroblasts demonstrated preferential growth on the highest-modulus hydrogel, and human umbilical vein endothelial cells demonstrated preferential growth on the lowest-modulus hydrogel investigated. Our studies suggest that the growth of multiple vascular cell types can be supported by PEG hydrogels and that different populations can be controlled by altering the mechanical properties of biomaterials.

Alternate JournalJ Biomed Mater Res A
Refereed DesignationRefereed